Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria.
Identifieur interne : 004770 ( Main/Exploration ); précédent : 004769; suivant : 004771Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria.
Auteurs : H. Shim [États-Unis] ; S. Chauhan ; D. Ryoo ; K. Bowers ; S M Thomas ; K A Canada ; J G Burken ; T K WoodSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 2000.
Descripteurs français
- KwdFr :
- Arbres (croissance et développement), Arbres (microbiologie), Dépollution biologique de l'environnement (MeSH), Hordeum (microbiologie), Mixed function oxygenases (génétique), Mixed function oxygenases (métabolisme), Pseudomonas (croissance et développement), Pseudomonas (génétique), Pseudomonas (métabolisme), Racines de plante (microbiologie), Recombinaison génétique (MeSH), Rhizobium (croissance et développement), Rhizobium (métabolisme), Trichloroéthylène (métabolisme), Triticum (microbiologie).
- MESH :
- croissance et développement : Arbres, Pseudomonas, Rhizobium.
- génétique : Mixed function oxygenases, Pseudomonas.
- microbiologie : Arbres, Hordeum, Racines de plante, Triticum.
- métabolisme : Mixed function oxygenases, Pseudomonas, Rhizobium, Trichloroéthylène.
- Dépollution biologique de l'environnement, Recombinaison génétique.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Hordeum (microbiology), Mixed Function Oxygenases (genetics), Mixed Function Oxygenases (metabolism), Plant Roots (microbiology), Pseudomonas (genetics), Pseudomonas (growth & development), Pseudomonas (metabolism), Recombination, Genetic (MeSH), Rhizobium (growth & development), Rhizobium (metabolism), Trees (growth & development), Trees (microbiology), Trichloroethylene (metabolism), Triticum (microbiology).
- MESH :
- chemical , genetics : Mixed Function Oxygenases.
- chemical , metabolism : Mixed Function Oxygenases, Trichloroethylene.
- genetics : Pseudomonas.
- growth & development : Pseudomonas, Rhizobium, Trees.
- metabolism : Pseudomonas, Rhizobium.
- microbiology : Hordeum, Plant Roots, Trees, Triticum.
- Biodegradation, Environmental, Recombination, Genetic.
Abstract
Indigenous bacteria from poplar tree (Populus canadensis var. eugenei 'Imperial Carolina') and southern California shrub rhizospheres, as well as two tree-colonizing Rhizobium strains (ATCC 10320 and ATCC 35645), were engineered to express constitutively and stably toluene o-monooxygenase (TOM) from Burkholderia cepacia G4 by integrating the tom locus into the chromosome. The poplar and Rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and were competitive against the unengineered hosts in wheat and barley rhizospheres for 1 month (colonization occurred at a level of 1.0 x 10(5) to 23 x 10(5) CFU/cm of root). In addition, six of these recombinants colonized poplar roots stably and competitively with populations as large as 79% +/- 12% of all rhizosphere bacteria after 28 days (0.2 x 10(5) to 31 x 10(5) CFU/cm of root). Furthermore, five of the most competitive poplar recombinants (e.g., Pb3-1 and Pb5-1, which were identified as Pseudomonas sp. strain PsK recombinants) retained the ability to express TOM for 29 days as 100% +/- 0% of the recombinants detected in the poplar rhizosphere expressed TOM constitutively.
DOI: 10.1128/aem.66.11.4673-4678.2000
PubMed: 11055909
PubMed Central: PMC92365
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Shim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, University of Connecticut, Storrs, Connecticut 06269-3222, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering, University of Connecticut, Storrs, Connecticut 06269-3222</wicri:regionArea><wicri:noRegion>Connecticut 06269-3222</wicri:noRegion></affiliation></author><author><name sortKey="Chauhan, S" sort="Chauhan, S" uniqKey="Chauhan S" first="S" last="Chauhan">S. Chauhan</name></author><author><name sortKey="Ryoo, D" sort="Ryoo, D" uniqKey="Ryoo D" first="D" last="Ryoo">D. Ryoo</name></author><author><name sortKey="Bowers, K" sort="Bowers, K" uniqKey="Bowers K" first="K" last="Bowers">K. Bowers</name></author><author><name sortKey="Thomas, S M" sort="Thomas, S M" uniqKey="Thomas S" first="S M" last="Thomas">S M Thomas</name></author><author><name sortKey="Canada, K A" sort="Canada, K A" uniqKey="Canada K" first="K A" last="Canada">K A Canada</name></author><author><name sortKey="Burken, J G" sort="Burken, J G" uniqKey="Burken J" first="J G" last="Burken">J G Burken</name></author><author><name sortKey="Wood, T K" sort="Wood, T K" uniqKey="Wood T" first="T K" last="Wood">T K Wood</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Hordeum (microbiology)</term><term>Mixed Function Oxygenases (genetics)</term><term>Mixed Function Oxygenases (metabolism)</term><term>Plant Roots (microbiology)</term><term>Pseudomonas (genetics)</term><term>Pseudomonas (growth & development)</term><term>Pseudomonas (metabolism)</term><term>Recombination, Genetic (MeSH)</term><term>Rhizobium (growth & development)</term><term>Rhizobium (metabolism)</term><term>Trees (growth & development)</term><term>Trees (microbiology)</term><term>Trichloroethylene (metabolism)</term><term>Triticum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (croissance et développement)</term><term>Arbres (microbiologie)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Hordeum (microbiologie)</term><term>Mixed function oxygenases (génétique)</term><term>Mixed function oxygenases (métabolisme)</term><term>Pseudomonas (croissance et développement)</term><term>Pseudomonas (génétique)</term><term>Pseudomonas (métabolisme)</term><term>Racines de plante (microbiologie)</term><term>Recombinaison génétique (MeSH)</term><term>Rhizobium (croissance et développement)</term><term>Rhizobium (métabolisme)</term><term>Trichloroéthylène (métabolisme)</term><term>Triticum (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mixed Function Oxygenases</term><term>Trichloroethylene</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Pseudomonas</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Pseudomonas</term><term>Rhizobium</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mixed function oxygenases</term><term>Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pseudomonas</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Arbres</term><term>Hordeum</term><term>Racines de plante</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hordeum</term><term>Plant Roots</term><term>Trees</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mixed function oxygenases</term><term>Pseudomonas</term><term>Rhizobium</term><term>Trichloroéthylène</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Recombination, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Recombinaison génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Indigenous bacteria from poplar tree (Populus canadensis var. eugenei 'Imperial Carolina') and southern California shrub rhizospheres, as well as two tree-colonizing Rhizobium strains (ATCC 10320 and ATCC 35645), were engineered to express constitutively and stably toluene o-monooxygenase (TOM) from Burkholderia cepacia G4 by integrating the tom locus into the chromosome. The poplar and Rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and were competitive against the unengineered hosts in wheat and barley rhizospheres for 1 month (colonization occurred at a level of 1.0 x 10(5) to 23 x 10(5) CFU/cm of root). In addition, six of these recombinants colonized poplar roots stably and competitively with populations as large as 79% +/- 12% of all rhizosphere bacteria after 28 days (0.2 x 10(5) to 31 x 10(5) CFU/cm of root). Furthermore, five of the most competitive poplar recombinants (e.g., Pb3-1 and Pb5-1, which were identified as Pseudomonas sp. strain PsK recombinants) retained the ability to express TOM for 29 days as 100% +/- 0% of the recombinants detected in the poplar rhizosphere expressed TOM constitutively.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11055909</PMID><DateCompleted><Year>2001</Year><Month>01</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>08</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>66</Volume><Issue>11</Issue><PubDate><Year>2000</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria.</ArticleTitle><Pagination><MedlinePgn>4673-8</MedlinePgn></Pagination><Abstract><AbstractText>Indigenous bacteria from poplar tree (Populus canadensis var. eugenei 'Imperial Carolina') and southern California shrub rhizospheres, as well as two tree-colonizing Rhizobium strains (ATCC 10320 and ATCC 35645), were engineered to express constitutively and stably toluene o-monooxygenase (TOM) from Burkholderia cepacia G4 by integrating the tom locus into the chromosome. The poplar and Rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and were competitive against the unengineered hosts in wheat and barley rhizospheres for 1 month (colonization occurred at a level of 1.0 x 10(5) to 23 x 10(5) CFU/cm of root). In addition, six of these recombinants colonized poplar roots stably and competitively with populations as large as 79% +/- 12% of all rhizosphere bacteria after 28 days (0.2 x 10(5) to 31 x 10(5) CFU/cm of root). Furthermore, five of the most competitive poplar recombinants (e.g., Pb3-1 and Pb5-1, which were identified as Pseudomonas sp. strain PsK recombinants) retained the ability to express TOM for 29 days as 100% +/- 0% of the recombinants detected in the poplar rhizosphere expressed TOM constitutively.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shim</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Connecticut, Storrs, Connecticut 06269-3222, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chauhan</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ryoo</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Bowers</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>S M</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Canada</LastName><ForeName>K A</ForeName><Initials>KA</Initials></Author><Author ValidYN="Y"><LastName>Burken</LastName><ForeName>J G</ForeName><Initials>JG</Initials></Author><Author ValidYN="Y"><LastName>Wood</LastName><ForeName>T K</ForeName><Initials>TK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>290YE8AR51</RegistryNumber><NameOfSubstance UI="D014241">Trichloroethylene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.12.-</RegistryNumber><NameOfSubstance UI="C093480">toluene ortho-monooxygenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001467" MajorTopicYN="N">Hordeum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011549" MajorTopicYN="N">Pseudomonas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011995" MajorTopicYN="Y">Recombination, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014241" MajorTopicYN="N">Trichloroethylene</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>10</Month><Day>31</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>10</Month><Day>31</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11055909</ArticleId><ArticleId 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Bowers</name><name sortKey="Burken, J G" sort="Burken, J G" uniqKey="Burken J" first="J G" last="Burken">J G Burken</name><name sortKey="Canada, K A" sort="Canada, K A" uniqKey="Canada K" first="K A" last="Canada">K A Canada</name><name sortKey="Chauhan, S" sort="Chauhan, S" uniqKey="Chauhan S" first="S" last="Chauhan">S. Chauhan</name><name sortKey="Ryoo, D" sort="Ryoo, D" uniqKey="Ryoo D" first="D" last="Ryoo">D. Ryoo</name><name sortKey="Thomas, S M" sort="Thomas, S M" uniqKey="Thomas S" first="S M" last="Thomas">S M Thomas</name><name sortKey="Wood, T K" sort="Wood, T K" uniqKey="Wood T" first="T K" last="Wood">T K Wood</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Shim, H" sort="Shim, H" uniqKey="Shim H" first="H" last="Shim">H. Shim</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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